calibration curves
In your next practical you will be constructing a calibration curve for glucose.... here is a little info about calibration curves... So, a calibration curve is a graph that shows the relationship between the concentration of something and the result of some analytical process.... In your case, your calibration curve this week will show the relationship between glucose concentration and absorbance. To create your calibration curve, you will make some solutions of glucose of known concentration using a serial dilution (post coming up soon on serial dilutions...) . You will then add a reagent to your glucose solutions (glucose oxidase) that reacts with the glucose to create a coloured compound - the more glucose there is, the greater the colour change will be. You will then be measuring the absorbance of light by each of your samples - more glucose will result in greater absorbance. Once you have done this, you will draw a wonderful graph of glucose con...